Description
Ciguatera fish poisoning (CFP) has been reported for many years in Rarotonga, Cook Islands, and has had the world’s highest reported incidence of this illness for the last 20 years. Following intensive sampling to understand the distribution of the causative organisms of CFP, an undescribed Gambierdiscus species was isolated from the Rarotongan lagoon. Gambierdiscus cheloniae sp. nov. has the common Gambierdiscus Kofoidian plate formula (except for a variability in the number of precingular plates in aberrant cells): Po, 30 , 600 (700), 6C?, 6 or 7S, 50 00 , 1p and 200 00 . The 20 plate is hatchet shaped and the dorsal end of 1p is pointed and the relatively narrow 1p plate. Morphologically G. cheloniae is similar to the genetically closely related species G. pacificus, G. toxicus and G. belizeanus, although smaller (depth and length) than G. toxicus. The apical pore plate varies from those of G. belizeanus and G. pacificus, which are shorter and narrower, and from G. toxicus, which is larger. G. cheloniae also differs from G. pacificus in the shape of the 20 plate. The description of this new species is supported by phylogenetic analyses using three different gene regions. G. cheloniae produced the putative maitotoxin-3 analogue, MTX-3, but neither maitotoxin or monitored ciguatoxin. Extracts of G. cheloniae were shown to be highly toxic to mice by intraperitoneal (i.p.) injection, although they were less toxic by gavage. It is possible that this species produces toxins other than putative MTX-3.
Enregistrements de données
Les données de cette ressource données d'échantillonnage ont été publiées sous forme d'une Archive Darwin Core (Darwin Core Archive ou DwC-A), le format standard pour partager des données de biodiversité en tant qu'ensemble d'un ou plusieurs tableurs de données. Le tableur de données du cœur de standard (core) contient 6 enregistrements.
2 tableurs de données d'extension existent également. Un enregistrement d'extension fournit des informations supplémentaires sur un enregistrement du cœur de standard (core). Le nombre d'enregistrements dans chaque tableur de données d'extension est illustré ci-dessous.
Cet IPT archive les données et sert donc de dépôt de données. Les données et métadonnées de la ressource sont disponibles pour téléchargement dans la section téléchargements. Le tableau des versions liste les autres versions de chaque ressource rendues disponibles de façon publique et permet de tracer les modifications apportées à la ressource au fil du temps.
Versions
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Comment citer
Les chercheurs doivent citer cette ressource comme suit:
Smith K F, Rhodes L, Verma A, Curley B G, Harwood D T, Kohli G S, Solomona D, Rongo T, Munday R, Murray S A (2024). A Gambierdiscus species (Dinophyceae) from Rarotonga, Cook Islands: Gambierdiscus cheloniae. Version 1.0. Southwestern Pacific Ocean Biogeographic Information System (OBIS) Node. Samplingevent dataset. https://nzobisipt.niwa.co.nz/resource?r=gambierdiscus_rarotonga&v=1.0
Droits
Les chercheurs doivent respecter la déclaration de droits suivante:
L’éditeur et détenteur des droits de cette ressource est Southwestern Pacific Ocean Biogeographic Information System (OBIS) Node. Ce travail est sous licence Creative Commons Attribution (CC-BY) 4.0.
Enregistrement GBIF
Cette ressource n'a pas été enregistrée sur le portail GBIF
Mots-clé
Samplingevent
Contacts
- Fournisseur Des Métadonnées ●
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- Personne De Contact
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- 98 Halifax Street East, Private Bag 2
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- 98 Halifax Street East, Private Bag 2
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- PO Box 123, Broadway
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- Chowder Bay Rd
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- 98 Halifax Street East, Private Bag 2
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- PO Box 123, Broadway
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- Private Bag
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- Author
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- 10 Bisley Road, Private Bag
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- PO Box 123
Couverture géographique
Rarotonga, Cook Islands
Enveloppe géographique | Sud Ouest [-21,273, -159,826], Nord Est [-21,211, -159,729] |
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Couverture temporelle
Date de début / Date de fin | 2014-11-01 / 2014-11-30 |
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Méthodes d'échantillonnage
Sampling was carried out in November 2014 at six selected sample sites: Muri Lagoon, Titikaveka Beach, adjacent to the Queen’s Representative residence (near Turoa Beach), the Papua Passage, adjacent to the Highland Paradise Cultural Centre and Black Rock Beach
Etendue de l'étude | Rarotonga, Cook Islands |
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Description des étapes de la méthode:
- Dinoflagellate cells were shaken from macro-algae into sealable plastic containers containing local sea water (approx. 500 mL each). Sub-samples were transferred to 50 mL Falcon tubes (BD Biosciences, USA) and germanium dioxide (approx. 1% final conc.) was added on arrival at the temporary laboratory (Muri Beachcomber Motel, Rarotonga, Cook Islands) in order to suppress diatom growth. Cells were isolated under a light microscope (Olympus CK2) using modified pickers (fine glass tubing with one end drawn to a fine tip under flame and the other end attached to fine silicone tubing so as suction can be applied) and transferred into 24-well tissue culture plates (Becton Dickinson, USA) containing sea water and f2 medium (Guillard, 1975; final conc. 5%) to support dinoflagellate growth. Uni-algal isolates were transported back to the laboratory at the Cawthron Institute, Nelson, New Zealand for culturing and determination of their DNA sequencing data, morphological characteristics and toxin production. On return to the Cawthron Institute, cultures were grown at 25 C +- 2 C with 40–70 mmol m-2 s-1 photon irradiance (12:12 h L:D). Selected clonal cultures were deposited in the Cawthron Institute Culture Collection of Micro-algae (CICCM).
Données de collection
Nom de la collection | Cawthron Institute, Nelson, New Zealand |
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Identifiant de collection | CAWD |
Citations bibliographiques
- Smith, K.F., Rhodes, L., Verma, A., Curley, B.G., Harwood, D.T., Kohli, G.S., Solomona, D., Rongo, T., Munday, R. and Murray, S.A., 2016. A new Gambierdiscus species (Dinophyceae) from Rarotonga, Cook Islands: Gambierdiscus cheloniae sp. nov. Harmful Algae, 60, pp.45-56.
Métadonnées additionnelles
marine, harvested by iOBIS
Identifiants alternatifs | https://nzobisipt.niwa.co.nz/resource?r=gambierdiscus_rarotonga |
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